Journal: bioRxiv

Article Title: Notch receptors involved in the choice between intestinal secretory and enterocytes and differentiation of Bestrophin 4 cells

doi: 10.64898/2025.12.17.694952

Figure Lengend Snippet: A. best4 RNA in situ hybridization (green) B. Nuclear mCherry (red-Notch activated reporter). C. Co-localization of best4 and nuclear mCherry. Arrows indicate best4 cells without nuclear mCherry expression. D. best4 RNA in situ hybridization (green) E. Pan-secretory cell marker AnnexinA4 (red-2F11 immunohistochemistry). F. Co-localization of best4 and pan secretory cell marker AnnexinA4. AnnexinA4 is also expressed in cells of the intrapancreatic ducts of the pancreas. Some of the intrapancreatic duct cells of the pancreas express AnnexinA4 to the side of the intestine with no best4 expression (arrows). G. Number of best4 cells (entire intestine) comparing DMSO control to DAPT treated embryos. H. Number of best4 cells (entire intestine) comparing WT to ascl1a mutants. DAPT n=9; ascl1a n=8; Scale bar: 20 μM. * p value ≤ 0.05; ** p value ≤ 0.01; *** p value ≤ 0.001

Article Snippet: Immunohistochemistry with AnnexinA4 (2F11 Abcam ab71286) and mCherry (Takara DsRed polyclonal antibody 632496) was incubated overnight followed by 45 minutes of PBST washes, incubation of secondary antibody (Invitrogen Alexa Fluor 488 and 594) for 2 hours, followed by 45 minutes wash PBST.

Techniques: RNA In Situ Hybridization, Expressing, Marker, Immunohistochemistry, Control